Mongoliibacter ruber gen. nov., sp. nov., a haloalkalitolerant bacterium of the family Cyclobacteriaceae isolated from a haloalkaline lake

A novel haloalkalitolerant, rod-shaped bacterium, designated strain YIM 4-4 T , was isolated from the surface water of the Dugerno lake, a haloalkaline lake in Inner Mongolia. The taxonomy of strain YIM 4-4 T was investigated by a polyphasic approach. Strain YIM 4-4 T was Gram-stain-negative, strictly aerobic, non-motile and formed red colonies. Optimal growth conditions were 28 °C, pH 8.0-11.0 and 0.5-2 % NaCl. The major respiratory quinone was menaquinone-7 (MK-7). The polar lipid profile was composed predominantly of phosphatidylethanolamine, six unidentified polar lipids, one phospholipid and one aminolipid. The predominant cellular fatty acids (>5 %) were iso-C 15 : 0 , iso-C 17 : 1 I/anteiso-C 17 : 1 B, iso-C 16 : 1 G, iso-C 17 : 0 3-OH, C 16 : 1 ω7 c /C 16 : 1 ω6 c and iso-C 16 : 1 . The genomic DNA G+C content was 43.0 mol%. 16S rRNA gene sequence analysis indicated that the members of the genera Cecembia , Fontibacter , Aquiflexum and Indibacter of the family Cyclobacteriaceae (phylum Bacteroidetes ) were the most closely related, with 16S rRNA gene sequence similarities ranging from 93.6 to 94.2 %. Other members of the family Cyclobacteriaceae showed sequence similarities < 93.0 %. On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain YIM 4-4 T represents a novel species of a new genus, for which the name Mongoliibacter ruber gen. nov., sp. nov. is proposed. The type strain is YIM 4-4 T ( = CCTCC AB 2012966 T  = DSM 27929 T ).