Saccharothrix stipae sp. nov., an actinomycete isolated from the rhizosphere of Stipa grandis

An actinomycete, strain D34 T , was isolated from a soil sample collected from the rhizosphere of Stipa grandis at Yunwu Mountain in Ningxia, north-west China. Strain D34 T showed highest 16S rRNA gene sequence similarity to Saccharothrix espanaensis DSM 44229 T (99.0 %), Saccharothrix texasensis NRRL B-16107 T (98.7 %) and Saccharothrix variisporea NRRL B-16296 T (98.6 %). The strain contained meso -diaminopimelic acid, alanine, glycine and glutamic acid as major cell-wall amino acids. Mannose, rhamnose and galactose were the characteristic whole-cell sugars. The fatty acid profile consisted predominantly of iso-C 15 : 0 , iso-C 16 : 0 , iso-C 16 : 1 , C 17 : 1 ω6 c , anteiso-C 17 : 0 and anteiso-C 15 : 0 . The phospholipid profile included phosphatidylethanolamine (typical of phospholipid pattern type II). Furthermore, a combination of some physiological and biochemical properties and low DNA-DNA relatedness values indicated that strain D34 T was differentiated from members of closely related species. On the basis of these phenotypic, genotypic and chemotaxonomic data, strain D34 T represents a novel species of the genus Saccharothrix , for which the name Saccharothrix stipae sp. nov. is proposed. The type strain is D34 T ( = JCM 30560 T  = ACCC19714 T ).