Acetobacter musti sp. nov., isolated from Bobal grape must

An acetic acid bacterium (strain Bo7 T ), obtained during a study of the microbial diversity of spontaneous fermentations of Bobal grape must, was subjected to a taxonomic study using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences allocated strain Bo7 T o the genus Acetobacter , and revealed Acetobacter aceti and Acetobacter oeni o be nearest neighbours (99.57 % 16S rRNA gene sequence similarity between strain Bo7 T and A. oeni CECT 5830 T , and 98.76 % between strain Bo7 T and A. aceti CECT 298 T ). Cells of strain Bo7 T are Gram-negative, motile rods, catalase-positive and oxidase-negative. The DNA G+C content of strain Bo7 T was 58.0 mol%. DNA-DNA hybridizations demonstrated that strain Bo7 T belongs to a single novel genospecies that can be differentiated from its nearest phylogenetic neighbours by the following phenotypic characteristics: no production of 5-keto-d-gluconic acid from d-glucose, growth with glycerol but not with methanol or maltose as sole carbon sources, and growth on yeast extract with 30 % d-glucose. The major fatty acid was C 18 : 1 ω7 c /C 18 : 1 ω6 c (summed feature 8; approx. 56 %); other fatty acids in significant amounts (>5 %) were C 16 : 0 2-OH (11 %), C 16 : 0 (7 %), C 14 : 0 2-OH (7 %) and C 14 : 0 3-OH/iso-C 16 : 1 I (summed feature 2; 6 %). The results obtained indicate that strain Bo7 T represents a novel species of the genus Acetobacter , for which the name Acetobacter musti sp. nov. is proposed. The type strain is Bo7 T ( = DSM 23824 T  = CECT 7722 T ).