Monashia flava gen. nov., sp. nov., an actinobacterium of the family Intrasporangiaceae

A novel actinobacterial strain, MUSC 78 T , was isolated from a mangrove soil collected from Peninsular Malaysia. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 78 T represented a novel lineage within the class Actinobacteria . Strain MUSC 78 T formed a distinct clade in the family Intrasporangiaceae and was related most closely to members of the genera Terrabacter (98.3-96.8 % 16S rRNA gene sequence similarity), Intrasporangium (98.2-96.8 %), Humibacillus (97.2 %), Janibacter (97.0-95.3 %), Terracoccus (96.8 %), Kribbia (96.6 %), Phycicoccus (96.2-94.7 %), Knoellia (96.1-94.8 %), Tetrasphaera (96.0-94.9 %) and Lapillicoccus (95.9 %). Cells were irregular rod-shaped or cocci and stained Gram-positive. The cell-wall peptidoglycan type was A3γ, with ll-diaminopimelic acid as the diagnostic diamino acid. The main cell-wall sugar was mannose and lower amounts of galactose and rhamnose were present. The predominant menaquinone was MK-8(H 4 ). The polar lipid profile consisted of phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipid. The predominant fatty acids were iso-C 15 : 0 , anteiso-C 15 : 0 and iso-C 16 : 0 . The DNA G+C content was 73.1 mol%. Based on this polyphasic study, MUSC 78 T exhibited phylogenetic and phenotypic differences from members of the genera of the family Intrasporangiaceae , and therefore a novel species of a new genus, Monashia flava gen. nov., sp. nov., is proposed. The type strain of Monashia flava is MUSC 78 T ( = DSM 29621 T  = MCCC 1K00454 T  = NBRC 110749 T ).