Parasediminibacterium paludis gen. nov., sp. nov., isolated from wetland

A novel orange-pigmented bacterial strain, designated HME6815T, was isolated from wetland in Jeju Island, Republic of Korea. The cells were Gram stain-negative, non-motile, strictly aerobic and rod-shaped. Optimal growth occurred at 30 °C and pH 7.0 on R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HME6815T formed a distinct phyletic lineage within the family Chitinophagaceae and was most closely related to members of the genera Sediminibacterium, Vibrionimonas, Hydrobacter, Hydrotalea and Asinibacterium with 92.3-94.3 % 16S rRNA gene sequence similarity. The major cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C13 : 0. The only respiratory quinone was MK-7. Polar lipid analysis revealed the presence of phosphatidylethanolamine, four unidentified aminolipids, one unidentified aminophospholipid and three unidentified polar lipids. The DNA G+C content was 38.4 mol%. On the basis of the evidence presented in this study, strain HME6815T represents a novel species of a new genus in the family Chitinophagaceae, for which the name Parasediminibacterium paludis gen. nov., sp. nov. is proposed. The type strain of the type species is HME6815T ( = KCTC 23736T = CECT 8010T).