Micromonospora oryzae sp. nov., isolated from roots of upland rice

An actinomycete strain, designated CP2R9-1 T , was isolated from root internal tissues of upland rice ( Oryza sativa ). Based on a polyphasic approach, strain CP2R9-1 T was characterized as a member of the genus Micromonospora. meso- Diaminopimelic acid and 3-OH-diaminopimelic acid were present in the cell-wall peptidoglycan. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, two unidentified phospholipids and four unidentified polar lipids. Predominant menaquinones were MK-9(H 4 ), MK-9(H 6 ) and MK-10(H 4 ). Whole-cell sugars consisted of ribose, xylose, arabinose and glucose. Phylogenetic analysis of the nearly complete 16S rRNA gene sequence suggested that strain CP2R9-1 T was closely related to Micromonospora haikouensis 232617 T (99.32 % similarity), Micromonospora carbonacea DSM 43168 T (99.18 %) and Micromonospora krabiensis MA-2 T (99.16 %). Strain CP2R9-1 T was distinct from its closest relatives based on low levels of DNA-DNA relatedness (21.3 ± 0.1-41.7 ± 0.7 %) and phenotypic differences. The results presented in this study showed that strain CP2R9-1 T represents a novel species of the genus Micromonospora , for which the name Micromonospora oryzae sp. nov. is proposed. The type strain is CP2R9-1 T ( = BCC 67266 T  = NBRC 110007 T ).