Open AccessBurkholderia sabiae sp. nov., isolated from root nodules of Mimosa caesalpiniifolia
Author(s) -
W.-M. Chen,
Sérgio Miana de Faria,
JyhHorng Chou,
Euan K. James,
Geoffrey N. Elliott,
Janet I. Sprent,
Cyril Bontemps,
J. Peter W. Young,
Peter Vandamme
Publication year - 2008
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijs.0.65816-0
Subject(s) - biology , 16s ribosomal rna , burkholderia , botany , strain (injury) , microbiology and biotechnology , root nodule , gene , genome , bacteria , nitrogen fixation , genetics , anatomy
Two rhizobial strains, Br3407(T) and Br3405, were isolated from nitrogen-fixing nodules on the roots of Mimosa caesalpiniifolia, a legume tree native to Brazil. On the basis of 16S rRNA gene sequence similarities, both strains were shown previously to belong to the genus Burkholderia. A polyphasic approach, including DNA-DNA hybridizations, pulsed-field gel electrophoresis of whole-genome DNA profiles, whole-cell protein analyses, fatty acid methyl ester analysis and extensive biochemical characterization, was used to clarify the taxonomic position of these strains further; the strains are here classified within a novel species, for which the name Burkholderia sabiae sp. nov. is proposed. The type strain is strain Br3407(T) (=LMG 24235(T) =BCRC 17587(T)).
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