Shewanella basaltis sp. nov., a marine bacterium isolated from black sand

A Gram-negative, motile, rod-shaped bacterium was isolated from black sand collected at Soesoggak, Jeju island, Korea. The strain, designated J83T, was able to grow in the presence of 5 % NaCl, at temperatures of 4-45 degrees C and over the pH range 5.5-9.5. The isolate reduced nitrate to nitrite and was positive for oxidase, catalase, alkaline phosphatase and leucine arylamidase. Strain J83(T) utilized malate, maltose, mannitol and glucose as sole sources of carbon. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain J83T belongs to the class Gammaproteobacteria and is related to species of the genus Shewanella. Strain J83T exhibited 97.8 % 16S rRNA gene sequence similarity to the type strain of Shewanella hafniensis. Based on DNA-DNA hybridization, the level of relatedness between strain J83T and S. hafniensis NBRC 100975T was 39.3 %. On the basis of phenotypic, genetic and phylogenetic data, strain J83T is considered to represent a novel species of the genus Shewanella, for which the name Shewanella basaltis sp. nov. is proposed. The type strain is J83T (=KCTC 22121T =JCM 14937T).