Marinobacterium litorale sp. nov. in the order Oceanospirillales

A bacterial strain named IMCC1877(T) was obtained from surface seawater collected near the coast of Deokjeok island (Yellow Sea), using a standard dilution-plating method. The strain was Gram-negative, chemoheterotrophic and facultatively anaerobic, requiring NaCl, and cells were motile rods with a single polar flagellum. Colonies on marine agar were very small (average diameter 0.1 mm). Based on 16S rRNA gene sequences, the most closely related species to strain IMCC1877(T) was Marinobacterium stanieri (93.7 % sequence similarity to the type strain). Phylogenetic analyses based on 16S rRNA gene sequences showed that this marine isolate belonged to the order Oceanospirillales and formed an independent phyletic line within the clade forming the genus Marinobacterium. The DNA G+C content of the strain was 60.7 mol% and the predominant constituents of the cellular fatty acids were C(18 : 1) omega 7c (36.6 %), C(16 : 1) omega 7c and/or iso-C(15 : 0) 2-OH (26.7 %) and C(16 : 0) (24.3 %). Based on the taxonomic data, only a distant relationship could be established between strain IMCC1877(T) and other Marinobacterium species; the strain therefore represents a novel species of the genus Marinobacterium, for which the name Marinobacterium litorale sp. nov. is proposed. The type strain is IMCC1877(T) (=KCTC 12756(T)=LMG 23872(T)).