Tamlana crocina gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae, isolated from beach sediment in Korea

A Gram-negative, aerobic, saffron-coloured marine bacterium, designated HST1-43(T), was isolated from beach sediment on the coast in Jeju, Korea, and its taxonomic status was established in a polyphasic study. 16S rRNA gene sequence analyses revealed that the isolate belonged to the family Flavobacteriaceae. The closest phylogenetic neighbours of strain HST1-43(T) were members of the genera Algibacter, Gaetbulibacter and Yeosuana, with levels of sequence similarity in the range 96.3-96.5 %. The isolate was non-motile and required sea salts or natural seawater for growth. The optimum temperature and pH ranges for growth were 25-30 degrees C and pH 6.1-8.1, respectively. MK-6 was the major menaquinone. The dominant cellular fatty acids were iso-C(15 : 0), C(18 : 0), C(16 : 0), iso-C(15 : 1) and iso-C(17 : 0) 3-OH. The DNA G+C content was 36.2+/-0.4 mol%. On the basis of phylogenetic distance and phenotypic characteristics, the isolate is considered to represent a novel genus and species in the family Flavobacteriaceae, for which the name Tamlana crocina gen. nov., sp. nov. is proposed. The type strain is HST1-43(T) (=KCTC 12721(T)=JCM 14021(T)).