Lutibacter litoralis gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from tidal flat sediment

A rod-shaped marine bacterium, designated strain CL-TF09 T , isolated from a tidal flat in Ganghwa, Korea, was characterized based on its physiological and biochemical features, fatty acid profile and phylogenetic position. 16S rRNA gene sequence analysis revealed a clear affiliation with the family Flavobacteriaceae . Strain CL-TF09 T showed the closest phylogenetic relationship with the genera Tenacibaculum and Polaribacter ; sequence similarities between CL-TF09 T and the type strains of Tenacibaculum and Polaribacter species ranged from 90·7 to 91·8 %. Cells of strain CL-TF09 T were non-motile and grew on solid media as yellow colonies. The strain grew in the presence of 1–5 % sea salts, within a temperature range of 5–30 °C and at pH 7–8. The strain had iso-C 15 : 0 3-OH (17·4 %), iso-C 15 : 0 (16·7 %), anteiso-C 15 : 0 (15·1 %) and iso-C 16 : 0 3-OH (13·4 %) as predominant fatty acids. The DNA G+C content was 33·9 mol%. Based on the physiological, fatty acid composition and phylogenetic data presented, strain CL-TF09 T is considered to represent a novel genus and species of the family Flavobacteriaceae , for which the name Lutibacter litoralis gen. nov., sp. nov. is proposed. The type strain is CL-TF09 T (=KCCM 42118 T =JCM 13034 T ).