Open AccessOceanobacillus oncorhynchi sp. nov., a halotolerant obligate alkaliphile isolated from the skin of a rainbow trout (Oncorhynchus mykiss), and emended description of the genus Oceanobacillus
Author(s) -
Isao Yumoto,
Kikue Hirota,
Yoshinobu Nodasaka,
Kenji Nakajima
Publication year - 2005
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijs.0.63483-0
Subject(s) - biology , halotolerance , rainbow trout , 16s ribosomal rna , microbiology and biotechnology , halophile , phylogenetic tree , bacteria , trout , gene , genetics , fishery , fish <actinopterygii>
A halotolerant, obligately alkaliphilic bacterium, R-2 T , was isolated from the skin of a rainbow trout ( Oncorhynchus mykiss ), a freshwater fish. The strain is Gram-positive, ferments several carbohydrates, is rod-shaped and motile by peritrichous flagella and produces ellipsoidal spores. The isolate grows at pH 9–10 but not at pH 7–8. This micro-organism grows in 0–22 % (w/v) NaCl at pH 10. Its major cellular fatty acids are iso-C 15 : 0 , anteiso-C 15 : 0 and anteiso-C 17 : 0 , the major isoprenoid quinone is MK-7 and the DNA G+C content is 38·5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicates that strain R-2 T is a member of the genus Oceanobacillus . DNA–DNA hybridization reveals low relatedness between the isolate and Oceanobacillus iheyensis (21·0 %). On the basis of phenotypic characteristics, phylogenetic data and DNA–DNA relatedness data, the isolate should be designated as a novel species, for which the name Oceanobacillus oncorhynchi sp. nov. is proposed. The type strain is R-2 T (=JCM 12661 T =NCIMB 14022 T ).