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Vibrio crassostreae sp. nov., isolated from the haemolymph of oysters (Crassostrea gigas)
Author(s) -
Nicole Faury,
Denis Saulnier,
Fabiano L. Thompson,
Mélanie Gay,
Jean Swings,
Frédérique Le Roux
Publication year - 2004
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijs.0.63232-0
Subject(s) - biology , vibrio , microbiology and biotechnology , 16s ribosomal rna , crassostrea , vibrionaceae , phylogenetic tree , hemolymph , bacteria , oyster , gene , genetics , botany , ecology
Polyphasic analysis of five new Vibrio isolates originating from the haemolymph of diseased cultured oysters is described. The new isolates were closely related to Vibrio splendidus, having 98 % 16S rRNA gene sequence similarity. gyrB phylogenetic analysis, fluorescent amplified-fragment length polymorphism (FAFLP) fingerprinting and DNA-DNA hybridization experiments clearly showed that the new isolates form a tight genomic group that is different from the currently known Vibrio species. It is proposed to accommodate these isolates in a novel species, Vibrio crassostreae sp. nov. (type strain LGP 7(T)=LMG 22240(T)=CIP 108327(T)). Phenotypic and chemotaxonomic features that differentiate V. crassostreae from other known Vibrio species include arginine dihydrolase, utilization and fermentation of various carbon sources, beta-galactosidase activity, NO(2) production and the presence of the fatty acids 14 : 0 iso and 16 : 0 iso.

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