Open AccessEnterococcus saccharominimus sp. nov., from dairy products
Author(s) -
Marc Vancanneyt,
Medana Zamfir,
Luc Devriese,
Karen Lefebvre,
Katrien Engelbeen,
Katrien Vandemeulebroecke,
M. Amar,
Luc De Vuyst,
Freddy Haesebrouck,
Jean Swings
Publication year - 2004
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijs.0.63193-0
Subject(s) - biology , enterococcus , enterococcus faecalis , 16s ribosomal rna , microbiology and biotechnology , intergenic region , enterococcus faecium , bacteria , gene , genetics , escherichia coli , genome , antibiotics
Four isolates, which were obtained from Belgian, Moroccan and Romanian dairy products, constituted a homogeneous but unidentified taxon after screening with whole-cell protein fingerprinting. Complete 16S rRNA gene sequence analysis classified representative strains in the genus Enterococcus. Highest sequence similarities of 98.6 and 98.0 % were obtained with the species Enterococcus sulfureus and Enterococcus saccharolyticus, respectively. Growth characteristics, biochemical features, tRNA intergenic length polymorphism analysis, DNA-DNA hybridization and DNA G+C contents of selected strains demonstrated that they represent a single, novel Enterococcus species. It differs phenotypically from other enterococci in characteristics commonly considered as typical of this genus: no growth in 6.5 % NaCl or 0.4 % sodium azide, and no acid production from a wide range of carbohydrates. The name Enterococcus saccharominimus sp. nov. is proposed for this novel species; the type strain (LMG 21727(T)=CCM 7220(T)) was isolated from contaminated pasteurized cow's milk.
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