Rhodoplanes oryzae sp. nov., a phototrophic alphaproteobacterium isolated from the rhizosphere soil of paddy

A Gram-stain-negative, rod-shaped, phototrophic bacterium, strain JA793(T), was isolated from rhizosphere soil of paddy. The strain was capable of growing phototrophically and chemotrophically. Bacteriochlorophyll-a and carotenoids of the spirilloxanthin series were present as photosynthetic pigments. The major fatty acid of strain JA793(T) was C18 : 1ω7c/C18 : 1ω6c (>65.7%), with minor amounts of C16 : 0, C16 : 1ω7c/C16 : 1ω6c, C20 : 2ω6,9c, C16 : 0 3-OH, C14 : 0 and C18 : 0 also present. Ubiquinone-10 and rhodoquinone-10 were present as primary quinones. Phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine were the major polar lipids, while minor amounts of amino lipids and unidentified lipids were also present. The G+C content of genomic DNA of strain JA793(T) was 68.7 mol%. 16S rRNA gene-based EzTaxon-e blast search analysis of strain JA793(T) indicated highest sequence similarity with members of the genus Rhodoplanes in the family Hyphomicrobiaceae of the class Alphaproteobacteria. Strain JA793(T) had high sequence similarity with Rhodoplanes elegans AS130(T) (98.6%), Rhodoplanes roseus 941(T) (98%), Rhodoplanes pokkaliisoli JA415(T) (97.5%) and Rhodoplanes piscinae JA266(T) (97.3%) and other members of the genus Rhodoplanes (<97%). However, strain JA266(T) was related by <59% (based on DNA-DNA hybridization) to Rhodoplanes elegans DSM 11907(T) ( = AS130(T)), Rhodoplanes roseus DSM 5909(T) ( = 941(T)), Rhodoplanes pokkaliisoli JA415(T) and Rhodoplanes piscinae JA266(T). The genomic information was well supported by phenotypic and chemotaxonomic data to classify strain JA793(T) as a representative of a novel species in the genus Rhodoplanes, for which the name Rhodoplanes oryzae sp. nov. is proposed. The type strain is JA793(T) ( = NBRC 109406(T) = KCTC 15260(T)).