Open AccessPseudorhodobacter aquimaris sp. nov., isolated from seawater, and emended description of the genus Pseudorhodobacter Uchino et al. 2002
Author(s) -
Yong Taek Jung,
Ki Hoon Oh,
Tae Kwang Oh,
JungHoon Yoon
Publication year - 2012
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijs.0.029769-0
Subject(s) - 16s ribosomal rna , biology , strain (injury) , phylogenetic tree , microbiology and biotechnology , gene , genetics , anatomy
A Gram-stain-negative, aerobic, motile, ovoid bacterial strain, designated HDW-19 T , was isolated from seawater of the west coast of Korea and subjected to a polyphasic taxonomic study. Strain HDW-19 T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2–3 % (w/v) NaCl. Bacteriochlorophyll a was not produced by strain HDW-19 T . Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain HDW-19 T clustered with Pseudorhodobacter ferrugineus IAM 12616 T , with which it shared 96.4 % similarity. A neighbour-joining phylogenetic tree based on gyrB gene sequences showed that strain HDW-19 T also clustered with the type strain of P. ferrugineus , sharing 83.0 % similarity. Strain HDW-19 T contained Q-10 as the predominant ubiquinone and C 18 : 1 ω7 c as the major fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, three unidentified aminophospholipids and two unidentified aminolipids. The DNA G+C content of strain HDW-19 T was 60.9 mol%. Differential phenotypic properties, together with phylogenetic distinctiveness, showed that strain HDW-19 T can be differentiated from P. ferrugineus . On this basis, strain HDW-19 T is considered to represent a novel species of the genus Pseudorhodobacter , for which the name Pseudorhodobacter aquimaris sp. nov. is proposed. The type strain is HDW-19 T ( = KCTC 23043 T = CCUG 58879 T ).