Nonomuraea rhizophila sp. nov., an actinomycete isolated from rhizosphere soil

A novel actinomycete, designated strain YIM 67092(T), was isolated from rhizosphere soil of the perennial vine Tripterygium wilfordii Hook. f. collected from Yunnan province, South-west China. The strain formed well differentiated aerial and substrate mycelia and grew in the presence of up to 7 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene showed that strain YIM 67092(T) belonged to the genus Nonomuraea, with highest sequence similarity to Nonomuraea rosea GW 12687(T) (99.0 %). Sequence similarities between strain YIM 67092(T) and other species of the genus Nonomuraea ranged from 97.8 % (Nonomuraea dietziae DSM 44320(T)) to 93.8 % (Nonomuraea kuesteri GW 14-1925(T)). Key morphological, physiological and chemotaxonomic characteristics of strain YIM 67092(T) were congruent with the description of the genus Nonomuraea. The G+C content of the genomic DNA was 69.3 mol%. Based on comparative analysis of physiological, biochemical and chemotaxonomic data, including low DNA-DNA hybridization results, strain YIM 67092(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea rhizophila sp. nov. is proposed. The type strain is YIM 67092(T) ( = CCTCC AA 209044(T)  = DSM 45382(T)).