Terrimonas aquatica sp. nov., isolated from a freshwater spring

A yellow-pigmented bacterial strain, designated RIB1-6(T), was isolated from a freshwater spring in Taiwan. Strain RIB1-6(T) was aerobic, Gram-negative, rod-shaped, non-motile and non-spore-forming. Growth occurred at 10-37 °C, at pH 7-8 and with 0-1 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain RIB1-6(T) belonged to the genus Terrimonas and its two closest neighbours were Terrimonas ferruginea ATCC 13524(T) and Terrimonas lutea DY(T) (16S rRNA gene sequence similarity 97.4 % and 93.5 %, respectively). Strain RIB1-6(T) contained iso-C(15 : 0) (33.4 %), iso-C(17 : 0) 3-OH (18.2 %), summed feature 3 (iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c; 14.7 %) and iso-C(15 : 1) (11.5 %) as the predominant fatty acids. The major isoprenoid quinone was MK-7. The DNA G+C content of strain RIB1-6(T) was 47.3 mol%. On the basis of the genotypic and phenotypic data, strain RIB1-6(T) represents a novel species in the genus Terrimonas, for which the name Terrimonas aquatica sp. nov. is proposed. The type strain is RIB1-6(T) (=BCRC 17941(T)=LMG 24825(T)).