Draconibacterium sediminis sp. nov., isolated from river sediment

A Gram-reaction-negative, facultatively anaerobic and rod-shaped bacterium, designated strain JN14CK-3(T), was isolated from surface sediment of the Jiulong River of China and was characterized phenotypically and phylogenetically. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain JN14CK-3(T) belonged to the genus Draconibacterium, with the highest sequence similarity (98.3%) to Draconibacterium orientale FH5(T). By contrast, strain JN14CK-3(T) shared low 16S rRNA gene sequence similarities ( < 91.0%) with other type strains. The sole respiratory quinone was MK-7. The polar lipids were phosphatidylethanolamine and several unidentified phospholipids and lipids. The major fatty acids were iso-C15:0, iso-C16:0, anteiso-C15:0, C17:0 2-OH, iso-C16:0 3-OH and iso-C17:0 3-OH. The G + C content of the genomic DNA was 40.9 mol%. The digital DNA-DNA hybridization value and average nucleotide identity (ANI) between strain JN14CK-3(T) and D. orientale FH5(T) were 34.2 ± 2.5% and 87.1%, respectively. The combined genotypic and phenotypic data showed that strain JN14CK-3(T) represents a novel species of the genus Draconibacterium, for which the name Draconibacteriumsediminis sp. nov. is proposed, with the type strain JN14CK-3(T) ( = MCCC 1A00734(T) = KCTC 42152(T)).