Pullulanibacillus pueri sp. nov., isolated from Pu'er tea

A novel Gram-stain-positive, aerobic, endospore-forming, rod-shaped bacterial strain YN3(T) was isolated from ripened Pu'er tea. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain belonged to the family Sporolactobacillaceae and was closely related to Pullulanibacillus naganoensis DSM 10191(T) (95.8% 16S rRNA gene sequence similarity) and Pullulanibacillus uraniitolerans DSM 19429(T) (95.4%). Growth of the strain was observed at 20-50 °C (optimum 30-37 °C), at pH 4.0-8.0 (optimum pH 5.0-6.0). The strain had a cell-wall type A1γ peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was menaquinone-7 (MK-7). The major fatty acids were anteiso-C15:0, anteiso-C17:0 and C18:1ω7c. The DNA G+C content of strain YN3(T) was 38.7 mol%. Strain YN3(T) could be differentiated from recognized species of the genus Pullulanibacillus based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA-DNA hybridization data. On the basis of polyphasic evidence from this study, Pullulanibacilluspueri sp. nov., is proposed, with strain YN3(T) ( = CGMCC 1.12777(T ) = JCM 30075(T)) as the type strain.