Dyadobacter jiangsuensis sp. nov., a methyl red degrading bacterium isolated from a dye-manufacturing factory

A Gram-stain-negative, non-motile, rod-shaped bacterial strain, L-1(T), which was capable of degrading methyl red was isolated from a dye-manufacturing factory in China. Phenotypic, chemotaxonomic and phylogenetic analyses established affiliation of the isolate to the genus Dyadobacter . Cells occurred in pairs in young cultures but became chains of coccoid cells in old cultures, and produced a flexirubin-like yellow pigment. Strain L-1(T) could not hydrolyse cellulose, and had a DNA G+C content of 51.3 mol%. The major cellular fatty acids were iso-C(15 : 0), C(16 : 1)ω5c, iso-C(17 : 0) 3-OH and summed feature 3 (C(16 : 1)ω7c and/or C(16 : 1)ω6c). C(16 : 0), iso-C(15 : 0) 3-OH and C(16 : 0) 3-OH were the other major fatty acid components. Comparative 16S rRNA gene sequence analysis showed that strainL-1(T) was most closely related to Dyadobacter fermentans DSM 18053(T) (99.2%), Dyadobacter soli JCM 16232(T) (98.9%) and Dyadobacter beijingensis CGMCC 1.6375(T) (98.7%). However, the new isolate exhibited relatively low levels of DNA-DNA relatedness with respect to JCM 16232(T) (41.2±1.8%), DSM 18053(T) (38.6±2.6%) and CGMCC 1.6375(T) (35.0±2.1%). Strain L-1(T) could also be differentiated from its closest phylogenetic relatives based on differences in several phenotypic characteristics. These data suggest that strain L-1(T) represents a novel species of the genus Dyadobacter , for which the name Dyadobacter jiangsuensis sp. is proposed. The type strain is L-1(T) (DSM 29057(T) = CGMCC 1.12969(T)).