Open AccessCharacterization of Bacillus thuringiensis and related bacteria by ribosomal RNA gene restriction fragment length polymorphisms
Author(s) -
Fergus G. Priest,
Denise A. Kaji,
Yoko Bomura Rosato,
Vanderlei Perez Canhos
Publication year - 1994
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.352
H-Index - 35
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/13500872-140-5-1015
Subject(s) - serotype , ribotyping , biology , bacillus thuringiensis , microbiology and biotechnology , ribosomal rna , bacillus cereus , pathogen , gene , genotype , bacteria , genetics
Ribosomal RNA gene restriction patterns have been determined for 43 strains of Bacillus thuringiensis representing 10 serovars and eight reference strains of B. anthracis, B. cereus and B. mycoides. Strains within a B. thuringiensis serovar produced highly related or identical ribotype patterns: in particular, 12 strains of serovar israelensis, five strains of serovar kurstaki, two strains of serovar galleriae and three strains of serovar aizawa produced ribotype patterns consistent with serotype designations. Moreover, variety tenebrionis (serotype 8a8b), a coleopteran pathogen, could be distinguished from the more common lepidopteran pathogens of this serotype (serovar morrisoni) by ribotyping. The correlation of ribotype patterns with serotype suggests a clonal population structure for B. thuringiensis.