Open AccessGrowth temperature regulates the induction of -lactamase in Pseudomonas fluorescens through modulation of the outer membrane permeation of a -lactam-inducing antibiotic
Author(s) -
Nicole Orange
Publication year - 1994
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 179
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/13500872-140-11-3125
Subject(s) - mezlocillin , periplasmic space , pseudomonas fluorescens , bacterial growth , pseudomonas , microbiology and biotechnology , chemistry , antibiotics , biology , bacterial outer membrane , biophysics , bacteria , chromatography , pseudomonas aeruginosa , biochemistry , escherichia coli , genetics , gene , piperacillin
The psychrotrophic bacterium, Pseudomonas fluorescens strain MFO, is more sensitive to the beta-lactam mezlocillin at a low growth temperature (i.e. 8 degrees C) than at a higher growth temperature (28 degrees C). An early effect of this antibiotic at all temperatures is bacterial filamentation, but this occurs later at 8 degrees C than at 28 degrees C, which suggests a lower permeability of the cell envelopes to mezlocillin at low growth temperature. beta-Lactamase production is later induced by mezlocillin, but the level of this induction also depends on the growth temperature, the overall induction being much less efficient at 8 degrees C. It is hypothesized that the periplasmic concentration of the drug might be too low at 8 degrees C to allow efficient beta-lactamase induction; this hypothesis was confirmed by the demonstration that beta-lactamase production is drastically enhanced in cells cultivated at 8 degrees C permeabilized for 10 min by Na-EDTA. In addition, induction kinetic curves display a marked dependence upon growth temperature. A rapid saturation was evident when mezlocillin concentrations were increased at 8 degrees C; this was not seen at 28 degrees C at up to 1000 micrograms mezlocillin ml-1. The results are discussed in terms of two different routes of drug permeation, depending on the growth temperature.