Open AccessImmunising potency of Leptospira interrogans serotype canicola after heat inactivation at different temperatures
Author(s) -
G. M. Painter,
H. C. Ellinghausen
Publication year - 1976
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-9-4-487
Subject(s) - potency , leptospira interrogans , serotype , microbiology and biotechnology , leptospira , virology , biology , in vitro , biochemistry
The immunogenicity of Leptospira interrogans serotype canicola suspensions inactivated by various degrees of heat exposure was examined in hamsters. No differences between leptospires killed at 50 degrees C and at 98 degrees C were shown. After exposure to 121 degrees C, suspensions retained their ability to protect against lethal infections but lost their ability to prevent leptospiruria. Tests with vaccines inactivated at or below 98 degrees C showed that the doses required for complete protection varied with the interval between vaccination and challenge. Larger doses were required to prevent the development of leptospiruria than to prevent death.