Open AccessA METHOD FOR DETERMINING THE UTILISATION OF GLUCOSE BY MYCOPLASMAS
Author(s) -
D. G. ff. Edward,
W. B. Moore
Publication year - 1975
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-8-3-451
Subject(s) - glucose oxidase , mycoplasma , biology , chemistry , microbiology and biotechnology , biochemistry , enzyme
A technique for measuring glucose utilisation by mycoplasmas, in which the disappearance of glucose is monitored by the glucose-oxidase method, is proposed as an alternative to the pH method. For maximum reliability the number of organisms in the test suspension must exceed a critical minimum value. Differences in efficiency of glucose utilisation were demonstrated between species of Mycoplasma and Acholeplasma.