Open AccessA Simple Single-step Immunoimmobilisation Method for the Detection of Salmonella in the Presence of Large Numbers of Other Bacteria
Author(s) -
Behzad Mohit,
Raza Aly,
Louis Bourgeois
Publication year - 1975
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-8-1-173
Subject(s) - salmonella , shigella , bacteria , agar , antiserum , microbiology and biotechnology , biology , enterobacteriaceae , citrobacter , escherichia coli , antigen , immunology , biochemistry , gene , genetics
A single-step disk immunoimmobilisation method for the detection of motile salmonellae is described. It combines in a single step the use of the motility of these bacteria in soft agar, their selective growth on Salmonella-Shigella media, and their immobilisation by polyvalent H antiserum. The method is shown to be 10 to 10,000 times more senstitive than a standard method for detection of Salmonella under the experimental conditons used. The time required for detection is shortened to 24-48 h.