Open AccessPCR identification of Pseudomonas aeruginosa and direct detection in clinical samples from cystic fibrosis patients
Author(s) -
Luiz Vicente Ferreira da Silva Filho,
Joséa Eduardo Levi,
Christi. O. Bento,
Scnia Regina Testa Da Silva Ramos,
Tatiana Rozov
Publication year - 1999
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-48-4-357
Subject(s) - pseudomonas aeruginosa , sputum , primer (cosmetics) , cystic fibrosis , microbiology and biotechnology , polymerase chain reaction , biology , bacteria , medicine , gene , chemistry , pathology , tuberculosis , genetics , organic chemistry
This report describes a PCR primer pair that targets the algD GDP mannose gene of Pseudomonas aeruginosa and produces a specific 520-bp PCR product useful for P. aeruginosa identification. This PCR assay was tested with 182 isolates of P. aeruginosa and 20 isolates of other bacterial species, and demonstrated 100% specificity and sensitivity. The test was also able to detect P. aeruginosa directly in clinical samples such as sputum or throat swabs obtained from cystic fibrosis patients. The combination of this primer with a universal bacterial primer, acting as a control to assess DNA quality in the sample, resulted in a robust PCR method that can be used for rapid P. aeruginosa detection.