Open AccessEstablishment of a model of penicillin-resistant Streptococcus pneumoniae pneumonia in healthy CBA/J mice
Author(s) -
Koji Takashima,
Kazuhiro Tateda,
Tetsuya Matsumoto,
Takeshi Ito,
Yuji Iizawa,
Masao Nakao,
Keizo Yamaguchi
Publication year - 1996
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-45-5-319
Subject(s) - streptococcus pneumoniae , microbiology and biotechnology , respiratory tract , pneumonia , penicillin , lung , immunology , biology , respiratory disease , infiltration (hvac) , respiratory system , medicine , antibiotics , physics , thermodynamics
Examination of strain differences in the susceptibility of mice to experimental respiratory tract infection with penicillin-resistant Streptococcus pneumoniae TUM19 revealed that a fatal infection model could be induced in immunocompetent CBA/J mice, but not in C3H/HeN, C57BL/6 or ICR mice. After intranasal instillation of c. 10(6) cfu of S. pneumoniae, the bacterial counts in the lungs of CBA/J mice increased from 10(5) to 10(7) cfu after 3-5 days, and gradually increased thereafter. The challenge organisms localised mainly in the lungs until 14 days after infection. Mice began to die c. 7 days after infection, and by 3 weeks most of the mice had died. Histopathologically, infiltration of neutrophils and lymphocytes around bronchi was observed from 1 day after infection, and fibrin deposition was seen in alveolar and bronchial spaces from 5 days. This model may be useful for investigating therapy of respiratory tract infection caused by penicillin-resistant S. pneumoniae because its pathological features resemble those observed in the human disease.