Open AccessCharacterisation of an enterotoxin-negative, cytotoxin-positive strain of Clostridium sordellii
Author(s) -
G. A. Green,
Véronique Schué,
Raymonde Girardot,
H. Monteil
Publication year - 1996
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-44-1-60
Subject(s) - enterotoxin , strain (injury) , microbiology and biotechnology , toxin , biology , polymerase chain reaction , gene , clostridiaceae , chemistry , biochemistry , escherichia coli , anatomy
In ileal loop assay, ELISA and anion-exchange column chromatography, Clostridium sordellii strain 6018 was shown to produce a cytotoxin, but no detectable enterotoxin. DNA sequence and polymerase chain reaction analyses indicated that the lack of enterotoxin activity is not due to a lack of gene transcription, but to lack of a major portion of the enterotoxin gene. This is the first characterisation of such a strain.