Open AccessCharacterisation of Burkholderia cepacia from cystic fibrosis patients living in Wales by PCR ribotyping
Author(s) -
H C Ryley,
L. Millar-Jones,
A. Paull,
Janet Weeks
Publication year - 1995
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-43-6-436
Subject(s) - ribotyping , burkholderia , polymerase chain reaction , microbiology and biotechnology , biology , taqi , cystic fibrosis , intergenic region , typing , burkholderia cepacia complex , restriction enzyme , restriction fragment length polymorphism , gene , genetics , bacteria , genome
Polymerase chain reaction (PCR) ribotyping detects differences in the intergenic spacer between the 16S and 23SrRNA genes. This method was applied to Burkholderia cepacia isolates from 16 Welsh cystic fibrosis (CF) patients attending three different clinics. Amplification of the intergenic spacer followed by an additional digestion step with TaqI restriction endonuclease identified seven distinct electrophoretic patterns among the patient isolates. Each of the seven patterns was distinct from that of the so called "epidemic strain" commonly isolated from patients attending clinics elsewhere in the UK. Two environmental isolates from the hospital clinics and four NCTC reference strains gave different patterns. The simplicity of the method lends itself to use in a general microbiological laboratory.