Typing of Aeromonas spp. by numerical analysis of immunoblotted SDS-PAGE gels | Zendy

R. Mulla | Zendy; S. Millership | Zendy

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Typing of Aeromonas spp. by numerical analysis of immunoblotted SDS-PAGE gels

Author(s) -

R. Mulla,

S. Millership

Publication year - 1993

Publication title -

journal of medical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.91

H-Index - 117

eISSN - 1473-5644

pISSN - 0022-2615

DOI - 10.1099/00222615-39-5-325

Subject(s) - typing , antiserum , polyclonal antibodies , serotype , aeromonas , microbiology and biotechnology , biology , bacteria , gel electrophoresis , polyacrylamide gel electrophoresis , virology , antibody , genetics , enzyme , biochemistry

One hundred and three isolates of Aeromonas spp., collected from both environmental sources and patients, were examined by SDS-PAGE of whole cells followed by immunoblotting with polyclonal rabbit antiserum raised against whole cells of A. sobria. All isolates were typable, yielding 15-20 well separated bands. Reproducibility of the technique was good and discrimination excellent, yielding 30 types amongst 103 isolates. Immunoblot type was not related to biochemical phenotype. Attempts to correlate immunoblot type with serotype were unsuccessful because only 42% of the strains tested could be serotyped.

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