Open AccessIron sources for Haemophilus ducreyi
Author(s) -
B. C. Lee
Publication year - 1991
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-34-6-317
Subject(s) - haemophilus ducreyi , lactoferrin , transferrin , siderophore , microbiology and biotechnology , bacterial outer membrane , biochemistry , bacteria , iron binding proteins , heme , haemophilus , chemistry , bovine serum albumin , biology , haptoglobin , deferoxamine , pasteurellaceae , enzyme , haemophilus influenzae , escherichia coli , gene , antibiotics , genetics , immunology
The ability of various haem- and non-haem-iron-containing compounds to support the growth of iron-limited cultures of Haemophilus ducreyi was assessed in a plate bioassay. Only haemin or the haem-containing proteins, bovine haemoglobin, human haemoglobin and bovine catalase, but not equine cytochrome C111, were capable of serving as the sole exogenous iron source. Complexes of haptoglobin-haemoglobin and haem-serum albumin retained the ability to function as iron substrates. In contrast, no growth was observed with FeCl3, human lactoferrin and human transferrin. Siderophore production was not detected with a universal chemical assay. Outer-membrane-protein profiles derived from iron-starved cultures revealed four iron-regulated polypeptides of 65, 50, 45.5 and 40.5 Kda. These results indicate that haem can supply the requisite iron for growth of H. ducreyi.