Open AccessCytotoxic activity of crude extracts of Bacteroides gingivalis
Author(s) -
Paul I. Eke,
Barbara E. Laughon,
Vincent O. Rotimi
Publication year - 1989
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-28-1-5
Subject(s) - cytotoxic t cell , bacteroides , bacteroidaceae , vero cell , microbiology and biotechnology , in vitro , trypsin , cell culture , bacteroides fragilis , cytotoxicity , bacteria , chemistry , biology , enzyme , biochemistry , antibiotics , genetics
The cytotoxic activities of culture supernates, crude cell extracts and cell-wall materials of Bacteroides gingivalis were investigated in vitro. Each component was cytotoxic to Vero cells and, to a lesser extent, Wi 38 cells. The cytotoxic agents had similar effects on the cell lines to butyric acid, propionic acid and a partially-purified trypsin-like protein extracted from a clinical isolate of B. gingivalis; the effects were eliminated by heat. Cytotoxic materials obtained from young cultures were more susceptible to heat than those from older cultures. The heart-labile substance inside and outside the bacterial cell in young cultures of B. gingivalis may contribute to its overall cytotoxic activity.