Open AccessEnterotoxin production by Salmonella typhimurium strains of different virulence
Author(s) -
Timothy S. Wallis,
W. Starkey,
J. Stephen,
S. J. Haddon,
Michael P. Osborne,
D. C. A. Candy
Publication year - 1986
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-21-1-19
Subject(s) - virulence , enterotoxin , salmonella , microbiology and biotechnology , biology , enterobacteriaceae , bacteria , escherichia coli , gene , genetics
Six strains of Salmonella typhimurium (TML, W118, LT7, SL1027, M206 and Thax-1) of known virulence and ability to induce fluid secretion when inoculated into the rabbit ileum were examined for enterotoxin production. Enterotoxic activity, assayed in the rabbit ileal-loop test, was detected in polymyxin-B extracts from all strains (with the possible exception of Thax-1) cultured for 6 h in casamino acid-yeast extract medium. The extracts were inactive in tissue-culture assays with CHO, Y-1 adrenal and Vero cells, and in the infant mouse assay for enterotoxin. There was no correlation between enterotoxigenicity in vitro and the ability of whole organisms to induce fluid secretion in vivo. The significance of these results in relation to salmonellosis is discussed.