Open AccessPolyacrylamide Gel Electrophoresis (Page) of Whole-Cell Proteins of Cutaneous Propionibacterium Species
Author(s) -
Katriordström
Publication year - 1985
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-19-1-9
Subject(s) - propionibacterium acnes , acne , polyacrylamide gel electrophoresis , propionibacterium , biology , gel electrophoresis , gel electrophoresis of proteins , microbiology and biotechnology , bacteria , dermatology , medicine , genetics , biochemistry , enzyme
Polyacrylamide gel electrophoresis (PAGE) was applied to the study of whole-cell proteins of cutaneous propionibacteria in an attempt to characterise possible protein patterns that may be typical for strains isolated from acne skin. Isolates were obtained from the faces of 33 individuals aged 7-16 years. Some of these subjects had apparently normal healthy skin, whereas others had acne vulgaris of varying severity. Twenty-five facial isolates of Propionibacterium acnes and eight of P. granulosum were studied. A further seven axillary strains of P. avidum were included for purely taxonomic interest. No particular protein pattern was characteristic of an isolate from acne skin; in fact the P. acnes strains from all sources appeared to be identical.