Open AccessThe isolation and immunochemical characterisation of a cell-wall carbohydrate and a membrane lipocarbohydrate antigen of group B streptococcus, type II
Author(s) -
C. G. Cumming,
Alister J. Hay,
P. W. Ross,
Ian R. Poxton
Publication year - 1983
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-16-1-53
Subject(s) - sialic acid , rhamnose , trichloroacetic acid , streptococcus , galactose , antigen , glucosamine , biochemistry , chemistry , carbohydrate , galactosamine , group a , microbiology and biotechnology , biology , bacteria , immunology , medicine , genetics
Two distinct carbohydrate antigens were isolated from the cell surface of group B streptococcus, type II. One antigen was extracted from SDS-purified cell walls by cold trichloroacetic acid and contained galactose, glucose, rhamnose, glucosamine and sialic acid in the approximate molar proportions 1.7:1.0:3.4:0.9:0.21 respectively. The serological activity of this polymer indicated that it is the group-specific antigen common to all group B streptococci. The second antigen was extracted by phenol from cell membranes and contained galactose, glucose, glucosamine, phosphorus and fatty acid in a molar ratio of 1.6:1.0:0.35:2.6:0.016 respectively. This antigen was shown to be specific for type II, group B streptococcus.