Open AccessDifferentiation of Subtypes within Leptospira Interrogans Serovars Hardjo, Balcanica and Tarassovi, by Bacterial Restriction-endonuclease DNA Analysis (Brenda)
Author(s) -
Anthony J. Robinson,
P. Ramadass,
Alison Lee,
R.B. Marshall
Publication year - 1982
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-15-3-331
Subject(s) - serotype , leptospira interrogans , restriction enzyme , biology , microbiology and biotechnology , leptospira , geography , genetics , dna
Various strains of Leptospira interrogans were compared by bacterial restriction-endonuclease DNA analysis (BRENDA). Field strains of serovar hardjo isolated from domestic animals in New Zealand, Australia and Northern Ireland were indistinguishable from one another but differed strikingly from the hardjo reference strain Hardjoprajitno. Similarly, field isolates of balcanica and tarassovi differed from their serovar reference strains, probably owing to a difference in epidemiological niche. Subdivision of these serovars into distinct subtypes as defined by BRENDA is therefore useful and justified. In contrast, analysis of serovars pomona, ballum and copenhageni shows that field and reference strains were identical, or differed only by a single band. It is suggested that BRENDA will overcome many of the problems associated with serological methods of identifying serovars and allow more precise definition of epidemiological relationships between strains and their hosts.