Open AccessPURIFICATION OF A PROTEIN TOXIN FROM CORYNEBACTERIUM ULCERANS
Author(s) -
Khachik Abrehem,
Iraj Zamiri
Publication year - 1980
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-13-4-587
Subject(s) - size exclusion chromatography , chromatography , polyacrylamide gel electrophoresis , toxin , chemistry , gel electrophoresis , ammonium , staining , microbiology and biotechnology , biochemistry , biology , enzyme , organic chemistry , genetics
Toxin from Corynebacterium ulcerans strain 378 was purified 66-fold by ammonium sulphate fractionation, dialysis, gel filtration on Ultrogel AcA22, ion-exchange chromatography on DEAE-cellulose and gel filtration on AcA54. On polyacrylamide-gel electrophoresis the purified material was homogeneous, staining for protein but not carbohydrate or lipid. The molecular weight of C. ulcerans toxin was 13000 as determined by SDS-polyacrylamide-gel electrophoresis and 15000 by gel filtration on AcA54.