Open AccessImmunogenicity and molecular characterisation of staphylococcal haemolysin (Plate XIII)
Author(s) -
T. H. Birkbeck,
D.D. Whitelaw
Publication year - 1980
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-13-2-213
Subject(s) - hemolysin , histidine , isoleucine , arginine , immunogenicity , antiserum , chemistry , amino acid , biochemistry , aspartic acid , biology , lysine , antibody , microbiology and biotechnology , leucine , virulence , genetics , gene
In rabbit antisera to staphylococcal delta haemolysin the haemolysin-neutralising activity was associated with the IgG fraction as well as with the alpha and beta lipoproteins. On an equal-weight basis, the neutralising capacity of specific antibody was not significantly greater than that of serum alpha or beta lipoproteins. Anti-alpha haemolysin was not detected in any of the anti-delta-haemolysin antisera. Amino-acid analysis of delta haemolysin showed that lysine, aspartic acid, threonine and isoleucine were the predominant amino acids present; histidine, arginine, proline, cysteine and tyrosine were absent. The molecular weight of delta-haemolysin in phosphate-buffered saline was approximately 210 000.