Open AccessTHE ISOLATION AND IDENTIFICATION OF BACTEROIDES SPP. FROM THE NORMAL HUMAN FAECAL FLORA
Author(s) -
B. I. Duerden
Publication year - 1980
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-13-1-69
Subject(s) - bacteroides fragilis , fusobacteria , biology , microbiology and biotechnology , bacilli , fusobacterium , bacteroides , flora (microbiology) , feces , bacteroides thetaiotaomicron , group b , bacteroidaceae , isolation (microbiology) , bacteria , 16s ribosomal rna , proteobacteria , medicine , genetics , antibiotics
Gram-negative anaerobic bacilli of the Bacteroides group were isolated on an enriched selective medium from specimens of faeces from 20 normal healthy adults and identified by conventional bacteriological methods. A heavy growth of Bacteroides spp. was obtained from all specimens and 10 representative colonies from each subject were identified. Most isolates (84%) belonged to the B. fragilis group. The commonest species identified in this group were B vulgatus and B. thetaiotaomicron (22% each), B. distasonis (18%) and the B. eggerthii/variabilis complex (14%), and only 9% were B. fragilis. B. vulgatus was isolated from 80% of subjects, B. thetaiotaomicron and B. distasonis from 70% each and the B. eggerthii/variabilis complex from 65%, but B. fragilis was detected in only 45% of specimens. Asaccharolytic species were isolated in smaller numbers from 14 (70%) subjects, but only five strains of the B. melaninogenicus/oralis group were isolated and fusobacteria were not detected.