Open AccessChemical and biological properties of a purified lymphoreticular-stimulating fraction of Corynebacterium parvum (Propionibacterium acnes type I) (Plate XXXV)
Author(s) -
C. Adlam,
David W. Reid,
Andrew J. Marshall,
P. Torkington
Publication year - 1979
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-12-4-429
Subject(s) - corynebacterium , peptidoglycan , corynebacterium parvum , propionibacterium acnes , cell wall , residue (chemistry) , chemistry , spleen , microbiology and biotechnology , cell , propionibacterium , biochemistry , biology , bacteria , immunology , genetics
A method is described in which washed whole cells of Corynebacterium parvum were chemically and enzymatically extracted to remove cytoplasm and cell-wall lipids. The resultant insoluble cell-wall residue possessed lympho-reticular stimulating properties as measured by their ability to increase spleen weight and protect against tumour-cell challenge. Analysis of the final product by chromatography and infrared spectroscopy has shown it to consist of carbohydrate and peptidoglycan, both of which appear to be necessary for the activities measured.