Comparison of several culture media used for studies on mycobacteriophages (Plates XII-XIV) | Zendy

C E Stager | Zendy; P R Gangadharam | Zendy

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Comparison of several culture media used for studies on mycobacteriophages (Plates XII-XIV)

Author(s) -

C E Stager,

P R Gangadharam

Publication year - 1978

Publication title -

journal of medical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.91

H-Index - 117

eISSN - 1473-5644

pISSN - 0022-2615

DOI - 10.1099/00222615-11-2-187

Subject(s) - lysis , mycobacterium smegmatis , mycobacterium , microbiology and biotechnology , strain (injury) , biology , serial dilution , bacteria , mycobacterium tuberculosis , genetics , tuberculosis , medicine , alternative medicine , pathology , anatomy

The value of RVA, N-1, 7H10, 7H11 and Sauton's media for studies on mycobacteriophage infeciton and lysis of mycobacteria was assessed. Experiments were made with mycobacteriophages BGI, BKI, CRI-3, G37, and LG, all of which lyse Mycobacterium smegmatis strain 607B, and with mycobacteriophage DS6A which lyses Mycobacterium tuberculosis strain H37Rv. The methods involved "direct lysis", the measurement of "routine test dilutions" and counts of plaque-forming units. It was found that N-1, 7H10 and 7H11 media gave better overall results than RVA medium for M. smegmatis strain 607B and its phages, and that RVA medium was generally the most useful for M. tuberculositems employed.

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