Open AccessEnterotoxicity of Aeromonas Hydrophila
Author(s) -
E Annapurna,
Sudip Sanyal
Publication year - 1977
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-10-3-317
Subject(s) - microbiology and biotechnology , feces , mucus , enterotoxin , aeromonas hydrophila , vibrionaceae , biology , vibrio , vibrio cholerae , bacteria , chemistry , escherichia coli , biochemistry , ecology , genetics , gene
Live cells and cell-free culture supernates of 50 strains of Aeromonas hydrophila isolated from diarrhoeic and healthy human faeces, drinking water, sewage, the river Ganges and faeces from domestic animals caused accumulation of fluid in ligated ileal loops of adult rabbits. The amount of fluid produced was comparable to that of a toxigenic strain of Vibrio cholerae. Three of the strains gave positive reactions only after two passages in ileal loops of rabbits. Inocula of about 10(3) viable cells and 0-25 ml of culture supernate caused fluid accumulation in the loops. The enterotoxic factor was inactivated at 60 degrees C for 20 min. and 65 degrees C for 10 min., was precipitated with ammonium sulphate and was non-dialysable; these results indicate the protein nature of the enterotoxin. An inoculum of 40 microgram of crude toxin caused as much fluid accumulation as larger inocula. The only histopathological change in the loops was depletion of mucus from the goblet cells.