Open AccessProperties of Phage-receptor Lipopolysaccharide from Pseudomonas morsprunorum
Author(s) -
Alan V. Quirk,
A. Sletten,
R. C. Hignett
Publication year - 1976
Publication title -
journal of general microbiology/journal of general microbiology
Language(s) - English
Resource type - Journals
eISSN - 2059-9323
pISSN - 0022-1287
DOI - 10.1099/00221287-96-2-375
Subject(s) - lipopolysaccharide , chlorosis , pseudomonas , chemistry , microbiology and biotechnology , inoculation , virulence , chromatography , electrophoresis , sodium , bacteria , biology , biochemistry , horticulture , genetics , organic chemistry , gene , endocrinology
Lipopolysaccharide (LPS) of Pseudomonas morsprunorum was extracted with hot phenol and purified by repeated centrifuging followed by either block electrophoresis or gel filtration. LPS from a virulent isolate exhibited specific phage inactivation (PI50 = 0.05 mug LPS ml-1), whereas LPS from an avirulent phage-resistant mutant did not. LPS was considered pure when a single band was detected following sodium dodecyl sulphate-cellulose acetate electrophoresis (pH 7.4). It was not phytotoxic when inoculated into cherry leaves at concentrations up to I mg ml-1, but produced weak chlorosis in bean and tobacco at 2 mg ml-1: no visible symptoms appeared after treatment with lower concentrations. The chemical composition of the LPS was partly determined.