Open AccessIsolation and Structure of an Extracellular Polysaccharide from Streptomyces sp. FERM-P1185
Author(s) -
Toshio Miyazaki,
Hideaki Yamada,
Juichi Awaya,
Satoshi Ōmura
Publication year - 1976
Publication title -
journal of general microbiology/journal of general microbiology
Language(s) - English
Resource type - Journals
eISSN - 2059-9323
pISSN - 0022-1287
DOI - 10.1099/00221287-95-1-31
Subject(s) - periodate , agar , mannose , chemistry , polysaccharide , asparagine , biochemistry , streptomyces , extracellular , isolation (microbiology) , agar plate , chromatography , microbiology and biotechnology , biology , bacteria , enzyme , genetics
A streptomycete strain (FERM-P1185), isolated from soil, secreted a slime on glucose-asparagine agar, and produced viscous growth in liquid media containing peptone as nitrogen source. A purified polysaccharide isolated from the culture broth was composed of glucose and mannose units (molar ratio 1-87:1). Periodate oxidation, Smith degradation, methylation analysis, infrared and 13c nuclear magnetic resonance spectra indicated that this mannoglucan had a linear structure consisting of alpha-1,3- and alpha-1,4-linked glucopyranose and mannopyranose units.