Open AccessMutagenic DNA Repair in Escherichia coli: Conditions for Error-free Filling of Daughter Strand Gaps
Author(s) -
Jórunn E. Eyfjörd,
M. H. L. Green,
B.A. Bridges
Publication year - 1975
Publication title -
journal of general microbiology/journal of general microbiology
Language(s) - English
Resource type - Journals
eISSN - 2059-9323
pISSN - 0022-1287
DOI - 10.1099/00221287-91-2-369
Subject(s) - daughter , escherichia coli , biology , ultraviolet light , dna , genetics , mutation , biophysics , microbiology and biotechnology , chemistry , photochemistry , gene , evolutionary biology
Two situations have been observed in which daughter strand gaps in DNA synthesized after exposure of excision-deficient Escherichia coli to ultraviolet light are filled but in which no mutations are formed as judged by loss of photoreversibility: (i) during the first 20 min of growth after u.v. irradiation, and (ii) when repair is allowed to occur in buffer. We suggest as an explanation that the majority of daughter strand gap-filling is error free and that mutations arise through a minor error-prone repair pathway which is inoperative under these conditions.