Open AccessManganese Mutagenesis in Yeast. A Practical Application of Manganese for the Induction of Mitochondrial Antibiotic-resistant Mutations
Author(s) -
Aleksandra Putrament,
Hanna Maria Baranowska,
Andrzej Ejchart,
Wiesława Prażmo
Publication year - 1975
Publication title -
journal of general microbiology/journal of general microbiology
Language(s) - English
Resource type - Journals
eISSN - 2059-9323
pISSN - 0022-1287
DOI - 10.1099/00221287-90-2-265
Subject(s) - manganese , yeast , mutagenesis , saccharomyces cerevisiae , mutant , mutagen , mutation , biology , mitochondrion , biochemistry , microbiology and biotechnology , gene , chemistry , genetics , dna , organic chemistry
When yeast cells were incubated for 4 to 8 h in yeast extract-peptone-glucose medium, pH 6, containing 8 mM-manganese, and then plated on selective media, there was a strong induction of antibiotic-resistant mutations. Indirect evidence suggests that practically all resistant mutants selected were of independent origin. The analysis of manganese-induced resistant mutants showed that most were extranuclear, while those tested showed recombination with known mitochondrial markers. Our results suggest that manganese can be considered as a mutagen which specifically induces mitochondrial mutations in Saccharomyces cerevisiae.