Open AccessMicrobodies in Methanol-grown Candida boidinii
Author(s) -
Hermann Sahm,
Rainer Roggenkamp,
Fritz Wagner,
W. Hinkelmann
Publication year - 1975
Publication title -
journal of general microbiology/journal of general microbiology
Language(s) - Uncategorized
Resource type - Journals
eISSN - 2059-9323
pISSN - 0022-1287
DOI - 10.1099/00221287-88-2-218
Subject(s) - alcohol oxidase , microbody , methanol , intracellular , biochemistry , ethanol , alcohol , carbon source , substrate (aquarium) , chemistry , mutant , biology , enzyme , organic chemistry , catalase , pichia pastoris , ecology , gene , recombinant dna
Intracellular structures were observed in Candida boidinii grown in a medium containing methanol as the sole source of carbon and energy; these structures were absent in the same organism grown in the presence of glucose or ethanol. These substrate-specific structures are ultrastructurally similar to microbodies. Studies with sphaeroplast and a mutant lacking alcohol-oxidase activity indicate that the alcohol may be located in these microbodies.