Open AccessStudies of Lactate Dehydrogenase of Mycoplasma mycoides var. mycoides
Author(s) -
B. A. Allsopp,
K. P. Matthews
Publication year - 1975
Publication title -
journal of general microbiology/journal of general microbiology
Language(s) - English
Resource type - Journals
eISSN - 2059-9323
pISSN - 0022-1287
DOI - 10.1099/00221287-88-1-58
Subject(s) - mycoplasma mycoides , isoelectric focusing , lactate dehydrogenase , enzyme , biochemistry , l lactate dehydrogenase , biology , isoelectric point , polyacrylamide gel electrophoresis , isozyme , microbiology and biotechnology , mycoplasma , chemistry
Polyacrylamide gel electrophoresis and isoelectric focusing techniques have been used to compare NAD-dependent L(plus) lactate dehydrogenases (LDH) from ten different strains of Mycoplasma mycoides var. mycoides. The enzymes were not distinguished from one another, or from normal bovine LDH 1 by these methods. The kinetic behaviour of LDH form M. mycoides (T1 vaccine strain) suggested that the enzyme could readily reduce pyruvate or oxidize lactate in a manner which, in vertebrates, requires two different isoenzymes.