Open Accessα-1,3 Glucan Synthesis Correlated with α-1,3 Glucanase Synthesis, Conidiation and Fructification in Morphogenetic Mutants of Aspergillus nidulans
Author(s) -
B. J. M. Zonneveld
Publication year - 2000
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 179
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/00221287-81-2-445
Subject(s) - laminarin , aspergillus nidulans , mycelium , conidiation , glucanase , glucan , spore , biology , mutant , conidium , biochemistry , chemistry , microbiology and biotechnology , enzyme , botany , gene
Summary: Nine mutants of Aspergillus nidulans differing in cleistothecia and/or conidia production were investigated. The strains were grown on four media with different levels of glucose (0.8, 3, and 4%) and nitrate (0.6 and 0.15%). The mycelia were analysed on the basis of the dry weight of the total mycelium and the alkali-soluble fraction (containing the α-1,3 glucan) as well as enzyme activities lytic to α-1,3 glucan, laminarin, and starch. Low quantities of α-1,3 glucan present on the third day are correlated with low α-1,3 glucanase activity and absence of cleistothecia formation on the sixth day. α-1,3 Glucan and cleistothecium formation seemed to be inversely related to conidiation.